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Procedimentos básicos para colheita de sangue em peixes. Infoteca-e
ISHIKAWA, M. M.; PÁDUA, S. B.; SATAKE, F.; PIETRO, P. S. de; HISANO, H..
O monitoramento do estado de saúde dos peixes é imprescindível para o manejo sanitário da produção, seja de grande ou pequeno porte. A hematologia clínica é uma ferramenta que permite a realização de diagnóstico de patologias e pode atuar como um indicador prognóstico das condições patológicas, especialmente quando se considera as alterações morfológicas nas células sanguíneas (SATAKE et al., 2009). Estas avaliações permitem identificar a resposta dos peixes, quando doentes, de maneira rápida, prática e de baixo custo
Tipo: Circular Técnica (INFOTECA-E) Palavras-chave: Manejo sanitário; Harvest.; Colheita; Peixe; Sangue.; Blood; Fish..
Ano: 2010 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/874164
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Colheita e processamento de amostras de sangue em suinos para fins de diagnostico. Infoteca-e
MORENO, A. M.; SOBESTIANSKY, J.; LOPEZ, A. C.; SOBESTIANSKY, A. A. B..
Objetivos da colheita de sangue em suinos; Caracteristicas da amostra; Principais anticoagulantes; Material necessario para colheita de sangue; Material; Identificacao de amostras; Colheita de sangue; Contencao do suino de acordo com a idade; Vias de acesso ao sangue; Veia cava cranial; Veia jugular; Veia cefalica; Vasos da orelha; Vasos da cauda; Seio orbital; Comparacao entre as tecnicas de colheita de sangue; Sequencia indicada para colheita de sangue por puncao venosa; Processamento de amostra; Armanezamento e viabilidade do sangue; Valores sanguineos de referencia; Tipos sanguineos dos suinos.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Coleta.; Sangue; Suíno.; Blood; Swine..
Ano: 1997 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/433735
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Change of osmoregulatory and hematological parameters in tilapia (Oreochromis niloticus) after exposure to sublethal mercury concentrations ArchiMer
Handayani, Kiki Syaputri; Soegianto, Agoes; Lignot, Jehan-hervé.
The effects of Hg exposure on blood parameters and gill physiology of tilapia (Oreochromis niloticus) were analyzed. Fish maintained in freshwater were exposed for 7 days (d) to sublethal mercury concentrations (0.1 and 1 mg/L). Blood serum osmolality (SO), sodium (Na+), potassium (K+) and chloride (Cl−) ionic concentrations, and hematological parameters were assessed after 1 up to 7 d of exposure. Serum osmolality and ionic concentrations of exposed fish appeared differently affected throughout the experimental period compared to the controls. Osmolality was reduced at the 2 tested concentrations but Na+ and Cl− contents were only altered at 1 mg/L of Hg after 1 d of exposure and values rapidly returned to the control values thereafter. K+ content was...
Tipo: Text Palavras-chave: Mercury; Tilapia; Osmoregulation; Serum ions; Blood; Aquatic toxicology.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00648/76033/76943.pdf
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Characterization of Blood Mononuclear Phagocytes in Phrynops hilarii (Chelonia Chelidae) International Journal of Morphology
Pitol,Dimitrius Leonardo; Issa,Joáo Paulo Mardegan; Caetano,Flávio Henrique; Lunardi,Laurelúcia Orive.
The localization of peroxidase activity in different cell regions is used as a criterion for the classification of the stage of maturation of mammalian mononuclear phagocytes with a positive peroxidase reaction indicating the presence of monoblasts, promonocytes, monocytes and macrophages. In this study it was evaluated the peroxidase activity of blood mononuclear phagocytes of this turtle detected at different stages of differentiation. The present observations suggest that, in turtles, the differentiation of mononuclear phagocytes occur in the blood circulation, in contrast to animals, where only are monocytes in circulating blood and macrophage differentiation occurs in other body compartments
Tipo: Journal article Palavras-chave: Mononuclear phagocytes; Blood; Turtle.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200021
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Morphological Characterization of the Leukocytes in Circulating Blood of the Turtle (Phrynops hilarii) International Journal of Morphology
Pitol,Dimitrius Leonardo; Issa,Joao Paulo Mardegan; Caetano,Flávio Henrique; Lunardi,Laurelúcia Orive.
The Phrynops hilarii specie of turtle has its characterization not well defined in the literature, it was proposed in this study the leukocyte characterization of the blood, stained by Leishman and analyzed under light and transmission electron microscope. It was not observe any cellular type with similar characteristics to neutrophils in mammalian group. We believed, based on the data obtained in this study that the heterophils have a morphofuncional analogy with another neutrophils belonged to mammalian group. This conclusion is being supported in many recent studies found in the literature
Tipo: Journal article Palavras-chave: Turtle; Morphology; Leukocytes; Blood.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400002
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Blood biochemical profile of the South American rattlesnake (Crotalus durissus terrificus) in captivity J. Venom. Anim. Toxins
TROIANO,J. C.; GOULD,E. G.; ALTHAUS,R.; MALINSKAS,G.; GOULD,J. A.; HEKER,J.; VIDAL,J. C.; AMANTINI,E.; SIMONCINI,C..
Blood samples were collected from 180 healthy specimens of the South American rattlesnake, Crotalus durissus terrificus, in captivity. All animals were in good clinical condition. Normal biochemical reference values were established for the following: total proteins, albumin, globulins, uric acid, creatinine, urea, glucose, triglycerides, cholesterol, total lipids, calcium, phosphorus, potassium, sodium, chloride, magnesium, GOT (AST), GPT (ALT), and alkaline phosphatase (ALP). Samples were obtained by venipuncture of the ventral tail vein. Values were compared with published data for Boidae, Elapidae, and Viperidae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Biochemistry Crotalus durissus terrificus captivity.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000200004
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Changes in the plasma levels of several bone markers in newborn calves during the first two days of life OAK
HATATE, Kaoru; SHINYA, Kazuhiko; MATSUO-SATO, Asako; SASAKI, Shuichi; DEVKOTA, Bhuminand; TAKAHASHI, Masahiro; HIRATA, Toichi; YAMAGISHI, Norio; 山岸, 則夫.
The fluctuations in the plasma levels of several bone markers were investigated in newborn calves. Experiment 1 monitored the postnatal changes in the plasma levels of tartrate-resistant acid phosphatase isoform 5b (TRAP5b), total alkaline phosphatase (t-ALP) and bone-specific alkaline phosphatase (BAP) in four calves. These markers increased significantly from 9−20 hr after the first colostrum-suckling compared with the values immediately after birth. Experiment 2 evaluated changes in the plasma TRAP5b, t-ALP, BAP and type I collagen cross-linked N-telopeptide (NTx) levels within 2 days post-birth in five calves with successful passive immunization via colostrum (non-deficient group) and five others with poor colostrum intake (deficient group). The...
Palavras-chave: Blood; Bone marker; Calf; Colostrum; Neonatal period.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4263
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Voluntary blood collection from the epidural sinus in two harbor seals (Phoca vitulina vitulina) OceanDocs
Sobrado, A..
La colección voluntaria de sangre en focas es una técnica díficil, el éxito depende de la habilidad del entrenador fundamentalmente. Requiere la relajación y cooperación total del animal. Se identifica aquí la secuencia de pasos que permiten esta actividad.
Tipo: Journal Contribution Palavras-chave: Haematology; Blood; Marine mammals.
Ano: 2011 URL: http://hdl.handle.net/1834/4163
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Studies on carbon-13 turnover in eggs and blood of commercial layers Rev. Bras. Ciênc. Avic.
Denadai,JC; Ducatti,C; Pezzato,AC; Carrijo,AS; Caldara,FR; Oliveira,RP.
This paper aimed at evaluating the influence of diets containing different isotopic values of carbon-13 turnover on the half-life of egg (yolk + albumen), yolk and albumen individually, and blood of poultry using delta‰13C isotopic variation. Commercial layers fed four experimental isocaloric and isonitrogenous diets (RC4, RC3, RMC4 and RMC3) containing different isotopic values, during an experimental period of 56 days. Turnover of the studied tissues was influenced by the experimental diets. Blood and albumen were more influenced by dietary treatments as compared to egg and yolk. The RMC3 diet induced better performance (better feed intake and higher egg production) due faster rate of carbon substitution than the RC4 diet, and lower half-life for egg...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Albumen; Carbon-13; Yolk; Blood; Egg; Comercial laying hens.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400008
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Egg traits and physiological neonatal chick parameters from broiler breeder at different ages Rev. Bras. Ciênc. Avic.
Luquetti,BC; Gonzales,E; Bruno,LDG; Furlan,RL; Macari,M.
This investigation was carried out to study egg traits (weight, specific gravity and eggshell thickness), and neonatal chick parameters (weight, blood, cardiac and lung parameters) from breeder at different ages. After hatching, neonatal chicks, male and female, from broiler breeder at three different ages (30, 45 and 60 weeks) were sacrificed and blood (red blood cell number, hematocrit, mean cell volume, blood viscosity, and haemoglobin), cardiac (right ventricle and total ventricle weights, cardiac index) and lung (mean pulmonary pressure, fresh relative lung weight) parameters were measured. No significant differences in eggshell thickness or specific gravity were observed in eggs from the three different breeder age. The incubated eggs and neonatal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler breeder age; Blood; Cardiac; Lung parameters; Neonatal chicks.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100002
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Feed restriction on performance and blood parameters of broilers fed diets with different sodium levels Rev. Bras. Ciênc. Avic.
Junqueira,OM; Fonseca,LEC; Araújo,LF; Duarte,KF; Araújo,CS da S; Rodrigues,EAp.
The experiment was conducted to evaluate the effect of feed restriction and different sodium levels in the diet on the performance and hematological values (erythrocyte, hemoglobin, hematocrit, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration and white blood cell) of broilers from 22 to 42 days of age. A completely randomized design was used in a 4 x 2 x 2 factorial (levels of sodium: 0.16%, 0.19%, 0.22% and 0.25%; with or without feed restriction; male and female), and two replicates of thirty broilers each. Sodium levels did not affect performance (p>0.05). Feed restriction resulted in increased feed intake and affected weight gain and feed conversion negatively (p<0.05). Better weight gain and feed conversion (p<0.05)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Broilers; Feed restriction; Nutrition; Sodium; Sodium bicarbonate.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000200003
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Addition of Amylase from Aspergillus Awamori to the Diet of Broiler Chickens Rev. Bras. Ciênc. Avic.
Morgado,HS; Cysneiros,CSS; Sousa,CM; Stringhini,JH; Ulhoa,CJ; Silva,AS; Fabino Neto,R; Freitas,PVDX; Oliveira,HP; Batista,LHC.
ABSTRACT Two experiments were performed to evaluate the hematological and blood biochemistry parameters, biometry of digestive organs, enzyme activities, protein content and absolute weight of the pancreas of broilers fed pre-starter and pre-starter diets supplemented or not with amylase from Aspergillus awamori. In total, 120 male Cobb chicks were housed in heated cages in each experiment. A completely randomized experimental design, with two treatments (feed with and without amylase) and six replicates per treatment of 10 birds each was applied. The data were subjected to analysis of variance using the F-test at 5% probability level. The dietary amylase addition did not affect hematological and blood biochemistry parameters and the biometry of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Chickens; Fungi; Nutrition.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400725
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Effects of addition of fluorine in diets differing in protein content on urinary fluoride excretion, clinical chemistry and thyroid hormones in calves R. Bras. Zootec.
Lohakare,Jayant; Pattanaik,Ashok Kumar.
In order to compare the effects of addition of fluorine (F) in diets differing in protein content on the urinary F excretion, blood profile and thyroid hormones, 30 crossbred calves (6-8 months) initially exposed to different protein levels were allotted into six groups in a 3 × 2 factorial design. The factors included three different levels of protein: normal (NP; 100%), low (LP; 75%), and high (HP; 125%) besides two levels of supplemental fluorine (as sodium fluoride) at 0 or 200 mg/kg diet. The animals were fed a wheat straw-based diet for 210 d. Feeding NP diets decreased urinary fluoride excretion, measured at 42 d intervals, compared with LP diets. Blood levels of hemoglobin and haematocrit, measured at 70 d intervals, were not affected by either...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Cattle; Concentrate; Fluorosis; Sodium fluoride; Urine.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013001000009
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Prediction of non-carcass components in cattle R. Bras. Zootec.
Costa e Silva,Luiz Fernando; Marcondes,Marcos Inácio; Valadares Filho,Sebastião de Campos; Ribeiro,André Mauric Frossard; Prados,Laura Franco; Nascimento,Fernando Barros; Gionbelli,Mateus Pies; Santos,Ronald Matos dos.
This study was conducted to develop equations to predict chemical composition of head, limbs, hide and blood in cattle. A database containing 335 animals from 10 trials, with 221 Nellore, 38 Nellore-Simmental and 76 Nellore-Angus (96 steers, 118 heifers and 121 bulls) animals was used. Models were constructed to estimate water, ether extract (EE), crude protein (CP), ash and macrominerals (calcium, phosphorus, magnesium, sodium and potassium) in the non-carcass parts of cattle. A stepwise procedure was conducted to determine the most significant variables within each model. Subsequently, a random coefficient model was used to construct the equation using studies as random effect, and sex and breed as fixed effects. The visceral fat was the most important...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Carcass; Ether extract; Hide; Organs and viscera; Protein.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000800015
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Use of different urea levels in the feeding of Alpine goats R. Bras. Zootec.
Barbosa,Julicelly Gomes; Costa,Roberto Germano; Medeiros,Ariosvaldo Nunes de; Queiroga,Rita de Cássia Ramos do Egypto; Batista,Ângela Maria Vieira; Medeiros,Geovergue Rodrigues de; Beltrão Filho,Edvaldo Mesquita.
The objective of this study was to evaluate intake, apparent digestibility, ingestive behavior and blood parameters of milking goats fed cactus pear (Opuntia ficus indica) and increasing urea levels in substitution to soybean meal. Ten multiparous Alpine goats with average weight of 31±6.0 kg were used and distributed into (5 × 5) Latin squares and assessed during five 17-day periods, receiving five treatments with the following substitution levels: 0; 5; 10; 15 and 20 g/kg of urea. Neutral detergent fiber (NDF), non-fiber carbohydrates, ether extract, organic matter and water intake values showed differences, whith average values ranging from 1.03 to 1.13; 0.12 to 0.14; 1.76 to 2.10 kg/day and 0.77 to 1.48 kg/day, respectively. The NDF and crude protein...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; DM intake; Milking goats; Rumination.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000700022
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Características hematológicas de teleósteos brasileiros: II. Parâmetros sangüíneos do Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) em policultivo intensivo Zoologia
Tavares-Dias,Marcos; Tenani,Rosilene A; Gioli,Leonardo D; Faustino,Christiane D.
Hematological parameters of freshwater fish Piaractus mesopotamicus (Holmberg, 1887) kept in an intensive polyculture system were determined. The correlations among these parameters as well as those between the parameters and the biometric data (total weight and standard length) were also analyzed. Erythrocytes count was 1.708±0.42 x 10(6)/µl, hemoglobin 6.6±2.0 g% of blood and hematocrit 32.5±4.6%, mean corpuscular volume (MCV) 209.8±69.5%, mean corpuscular hemoglobin (MCH) 40.2±5.6 pg and mean corpuscular hemoglobin concentration (MCHC) 21.4±8.4%. The percentage of defense blood cells including leucocytes and thrombocytes, was studied. The percentage of thrombocytes was 61.4±10.0%, lymphocytes 21.7±1.8%,neutrophils 10.4±11.0%, monocytes 4.7±3.3% and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Piaractus mesopotamicus; Blood; Erythrocytes; Freshwater fish; Leucocytes; Thrombocytes.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000200008
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Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion Acta Amazonica
Tavares-Dias,Marcos; Oliveira-Júnior,Antônio Aderson; Marcon,Jaydione Luiz.
The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). Direct WBC counts using the Natt and Herrick method showed limitations, which are discussed here. The WBC and TT counts using 1,000 erythrocytes from blood smears are not recommended for Amazon turtles nor other reptilian species, since wide variation in counts can be observed. Estimation methods for determining WBC and TT based on 2,000 erythrocytes of blood...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon turtles; Blood; Leukocytes; Podocnemis expansa; Thrombocytes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000200020
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Comparative study on hematological parameters of farmed matrinxã, Brycon amazonicus Spix and Agassiz, 1829 (Characidae: Bryconinae) with others Bryconinae species Acta Amazonica
Tavares-Dias,Marcos; Affonso,Elizabeth Gusmão; Oliveira,Sarah Ragonha; Marcon,Jaydione Luiz; Egami,Mizue Imoto.
The aim of this study was not only to determine the red blood cells parameters, thrombocyte and leukocyte counts in farmed Brycon amazonicus (matrinxã), to compare these parameters among Bryconinae species from literature, and also to investigate the presence of special granulocytic cells in these fish. The results of the blood cells parameters here established for farmed B. amazonicus, a species of great economic importance in Brazilian aquaculture, could help a better understanding of the blood features in natural populations of this Amazon species. Blood parameters varied between Bryconinae species investigated, mainly the red blood cell counts, hemoglobin, hematocrit and mean corpuscular volume (MCV). The presence of the blood granulocytes, neutrophils...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leukocytes; Freshwater fish; Blood; Amazon; Brycon amazonicus; Bryconinae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000400026
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HEMATOLOGYCAL AND BLOOD BIOCHEMISTRY PARAMETERS OF CAPTIVE BIG-HEADED AMAZON RIVER TURTLES, Peltocephalus dumerilianus (TESTUDINES: PODOCNEMIDIDAE) Acta biol.Colomb.
ANSELMO,Neiana Pereira; FRANÇA,Mônica Ferreira de Lima; SANTOS,Mareio Quara de Carvalho; PANTOJA-LIMA,Jackson; SILVA,Cleide Karoline Pereira da; ARIDE,Paulo Henrique Rocha; OLIVEIRA,Adriano Teixeira de.
ABSTRACT The determination of hematological values is used to obtain knowledge about the health conditions of animal species. The big-headed Amazon River turtles, (Peltocephalus dumerilianus) are considered one of the least known testudine species concerning their biology and health status. Herein, we determined the hematological and plasma biochemical parameters of17 (eight males and nine females) adult P. dumerilianus to provide reference interval values for clinically healthy individuals. We collected the blood samples by puncturing the femoral vein using long heparinized hypodermic syringes. Sexual dimorphism for individuals was determined by external observation of the shape of the plastron. The average values obtained for the ten hematological and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon; Blood; Hematology; Physiology; Reptiles.
Ano: 2021 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2021000200207
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Protein, energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum Animal Sciences
Oliveira, Wagner Dias Coelho de; Silva, Tairon Pannunzio Dias e; Bezerra, Leílson Rocha; Araújo, Marcos Jácome de; Edvan, Ricardo Loiola; Lopes Oliveira, Ronaldo.
The aim of this study was to evaluate the protein energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum. Fifteen multiparous cows with 4 ± 1 years of age and live weight of 400 ± 50 kg were used at different stages (non-pregnant, in the initial, middle and late pregnancy, at birth, one day postpartum, 30 and 60 days postpartum). Blood collections were performed every 30 days and assayed for the following blood biomarkers: Protein (total proteins, albumin, urea and creatinine), energetic (cholesterol, triglycerides, glucose and beta hydroxybutyrate), mineral (calcium, phosphorus, magnesium) and enzymatic (alkaline phosphatase and aspartate aminotransferase). Calcium had the lowest concentrations (p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biochemistry; Blood; Ketone bodies; Metabolites; Physiology. biochemistry; Blood; Ketone bodies; Metabolites; Physiology..
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/49022
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